二氢青蒿素对大鼠牛Ⅱ型胶原蛋白诱导关节炎模型作用的初步研究

【摘要】目的建立牛Ⅱ型胶原蛋白(BCⅡ)诱导的大鼠关节炎(collagen Ⅱ-induced arthritis，CIA)动物模型，研究二氢青蒿素对大鼠关节炎的影响。方法选用雄性Wistar大鼠，在模型组(n=19)大鼠尾根及脊背部皮内注射用弗氏完全佐剂乳化的BCⅡ 0.3 mg，第15 d以同样的剂量在同样的部位加强免疫1次；正常对照组(n=5)以生理盐水按同样的方法和剂量注射。第52 d，将模型组分为单纯模型组（模型空白组，n=7）、二氢青蒿素组〔二氢青蒿素1.5 mL/（kg·d)，1 mL含20 mg二氢青蒿素,灌胃；n=12〕。治疗28 d后观察大鼠的关节炎表现。结果模型组大鼠关节肿胀明显，而正常对照组关节无炎性红肿。二氢青蒿素组：关节肿胀减轻，组织病理检查见只有少量炎性细胞浸润，少量纤维结缔组织和毛细血管增生，关节腔较干净，没有明显的骨质破坏。治疗28 d后，模型空白组关节炎指数较治疗前和正常对照组增加（P<0.05）；二氢青蒿素组与治疗前比较差异无统计学意义（P>0.05），仍高于正常对照组（P<0.05）。治疗28 d后，模型空白组病理分级高于正常对照组（P<0.05)，二氢青蒿素组低于模型空白组（P<0.05）。结论初步实验表明二氢青蒿素有减轻Ⅱ型胶原诱导的大鼠关节炎病变作用。

英文摘要:

【Abstract】 Objective To study the effects of dihydroartemisinin on collagen-induced arthritis (CIA) in rats model.Methods CIA was induced in Male Wistar rats by intradermal injection of emulsion of 0.3 mg type Ⅱ collagen in Freund’s completead juvant (CⅡ/FA) at the base of the tail and repeated one times after 15 days. The normal control rats received the injection of the same volume of saline 52 days later, the CIA rats were divided into dihydroartemisinin treated group, saline treated group, in which the rats were subjected to the treatment of dihydroartemisinin 30 mg/(kg·d) or the same volume of saline for 28 days, then the effects of the treatments on arthritis were evaluated..Results Compared with the normal rats, the joints of non-treated CIA rats were obviously swollen. After the treatment of dihydroartemisinin, the joints of CIA rats appeared less inflammation cell erosion, less proliferation of fibrous connective tissue and blood capillary, more clear, articular cavity and no bone erosion. Conclusion This preliminary study indicated dihydroartemisinin may have the effects to relieve CIA in rats.

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