LIU Liu, LIU Lin, LIANG Li-chuan, et al.Effects of IL-1β Pretreated Adipose Tissue-derived Mesenchymal Stem Cells on VEGF Secretion and Macrophage M2 Polarization.Journal of Sichuan University (Medical Science Edition),2019;50(2):171-176
Effects of IL-1β Pretreated Adipose Tissue-derived Mesenchymal Stem Cells on VEGF Secretion and Macrophage M2 Polarization
ObjectiveTo investigate the effects of interleukin (IL)-1β preteated adipose tissue-derived mesenchymal stem cells (ADMSCs) on vascular endothelial growth factor (VEGF) secretion and macrophages M2 polarization. MethodsAfter IL-1β pretreated ADMSC for 24 h, the expression of cyclooxygenase-2 (COX-2) was detected using Western blot, and the secretions of prostaglandin E2 (PGE2) and VEGF were measured by ELISA method. Conditioned media collected from ADMSCs was used for culturing PMA-U937 macrophages for 72 h, and the expressions of CD163 and IL-10 mRNAs were detected using qRT-PCR. After inhibition of COX-2 expression in ADMSCs by Lentivirus silencing, secretion of VEGF and CD163 and IL-10 mRNA expressions were detected. ResultsIL-1β pretreating ADMSCs increased the expression level of COX-2, enhanced PGE2 and VEGF secretions (P<0.05). In addition, the mRNA levels of CD163 and IL-10 were upregulated when ADMSCs were pretreated by IL-1β in PMA-U937 cells. After downregulation of COX-2 in ADMSCs, the secretion of VEGF was suppressed, and the mRNA levels of CD163 and IL-10 were also significantly down-regulated. ConclusionIL-1β pretreatment enhanced the secretion of PGE2 and VEGF, and induced macrophage M2 polarization, which depended on COX-2-PGE2 signal pathway.
