YANG Yan, LIU Xing-ren, JIN Zhao.Effects of Interference with UCA1 and Inhibition of miR-185-5p on Activation, Autophagy and Survival of β-Catenin Pathway in Non-small Cell Lung Cancer.Journal of Sichuan University (Medical Science Edition),2019;50(2):157-163
Effects of Interference with UCA1 and Inhibition of miR-185-5p on Activation, Autophagy and Survival of β-Catenin Pathway in Non-small Cell Lung Cancer
  
Key words:LncRNA UCA1miR-185-5pA549 cellsβ-Catenin
Author NameAffiliation
YANG Yan, LIU Xing-ren, JIN Zhao 四川省医学科学院·四川省人民医院 呼吸与危重症医学科 
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Abstract:
      ObjectiveTo investigate the effect on β-Catenin pathway by lncRNA urothelial carcinoma associated 1 (UCA1) targeting regulated miR-185-5p in human lung adenocarcinoma A549 cell line. MethodsA549 cell was selected as the study model and were divided into four groups, blank control group, sh-scramble negative control group (sh-scramble), sh-UCA1 interference group (sh-UCA1), miR-185 inhibitor group (miR-185 inhibitor) and sh-UCA1+ inhibitor group (sh-UCA1+inhibitor). The proliferation-, apoptosis- and autophagy- related protein levels were determined by Western blot. qRT-PCR was employed to detect the mRNA levels of UCA1 and miR-185-5p. The relationship between lnRNA UCA1 and miR-185-5p was validated by bioinformatics analysis and luciferase reporter system assays. BrdU staining was used to detect the cell growth, and immunofluorence staining was performed to measure the content of LC3+ cells. Resultssh-UCA1 significantly decreased UCA1 expression and increased miR-185-5p expression in A549 cells, and inhibited the cell growth and autophagy, while promoted the cell apoptosis (P<0.01). Bioinformatics analysis and luciferase reporter system assays demonstrated that lncRNA UCA1 and miR-185-5 can combine effectively, indicating that they have a targating relationship. sh-UCA1 also significantly inhibited the protein levels of β-Catenin/TCF-4, Beclin 1 and LC3 Ⅱ, and decreased the cell growth and autophagy by the miR-185-5p; and down-regulated the LC3 expression (P<0.01). ConclusionThe effect of UCA1 inhibition for miR-185-5p was decreased by lncRNA UCA1 inference, and released the β-Catenin/TCF-4, Beclin 1 and LC3 Ⅱ, and further reduced the autophagy and growth in A549 cells.
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