MU Qi-ming, HE Wei, HOU Gui-min, et al.Interference of Lumican Regulates the Invasion and Migration of Liver Cancer Cells.Journal of Sichuan University (Medical Science Edition),2018;49(3):358-363
Interference of Lumican Regulates the Invasion and Migration of Liver Cancer Cells
  
Key words:Liver cancer lumican shRNA Invasion Migration ERK1/JNK pathway
Author NameAffiliation
MU Qi-ming, HE Wei, HOU Gui-min, et al 航空工业三六三医院 肝胆胰外科 
Hits: 2180
Download times: 0
Abstract:
      Objective To explore the effect of silencing lumican on the invasion and migration of liver cancer cells. Methods Lumican was silenced by shRNA in liver cancer cells (HepG2 and MHCC97H). The mRNA levels of lumican were detected by qRT-PCR. Cell invasion was measured by Transwell. Cell migration was tested via wound healing. The protein levels of lumican, MMP-9, VEGF, ERK1, JNK, p-ERK1 and p-JNK were measured by Western blot. Results Liver cancer cells (HepG2 and MHCC97H) had higher levels of mRNA and protein of lumican compared with normal hepatocyte L02 (P<0.01). shRNA lowered the levels of mRNA and protein of lumican (P<0.01), and weakened the invasion and migration of cancer cells (P<0.01). The expressions of MMP-9 and VEGF decreased with the shRNA silence (P<0.01). shRNA also reduced the protein level of p-ERK1 and p-JNK (P<0.01). Conclusion Silencing lumican by shRNA attenuates the invasion and migration of liver cancer cells via inhibiting the activation of ERK1/JNK pathway.
View Full Text  View/Add Comment  Download reader
Close