针刺足三里对COPD大鼠血浆多巴胺和肺功能的影响

目的观察针刺足三里对慢性阻塞性肺疾病（COPD）模型大鼠的血浆多巴胺、炎症因子、肺功能及肺组织形态学的影响，探讨多巴胺在针刺调控COPD慢性炎症机制中的作用。方法32只SD 大鼠随机分为正常对照组、模型组、假针刺组、针刺组（n=8）。后3组大鼠采用香烟烟熏和脂多糖复合法诱导8周制备COPD模型。自第7周起，烟熏前针刺组电针大鼠双侧足三里穴（ST 36）、假针刺组电针双侧足部非穴位处，30 min/次，1天1次，连续2周；正常对照组和模型组大鼠不做电针治疗。比较治疗后各组大鼠血浆多巴胺及炎症因子〔肿瘤坏死因子-α（TNF-α）、白介素-1β、6、8（IL-1β、IL-6、IL-8）〕质量浓度，进行肺功能〔肺容积（TLC）、功能残气量（FRC）、第50微秒及100微秒用力呼气量与用力肺活量之比（FEV₅₀/FVC, FEV₁₀₀/FVC）、总气道阻力（RL）和肺动态顺应性（Cdyn）〕、肺泡总面积与肺组织面积之比（A/t）及肺泡灌洗液（BALF）内细胞数比较，并将上述指标进行Pearson相关分析。结果针刺组炎症因子、肺功能、A/t及BALF内细胞数均有不同程度改善，而血浆多巴胺质量浓度高于其它组。与模型组相比，针刺组TNF-α和IL-1β质量浓度、A/t及BALF内细胞数、肺功能（FEV₅₀/FVC，FEV₁₀₀/FVC，RL，Cdyn）的差异有统计学意义（P<0.05），且针刺组优于假针刺组。Pearson相关分析表明：肺功能FRC、RL、Cdyn与炎症因子IL-1β、IL-6、IL-8、TNF-α及A/t均有关（P<0.001）, 而TLC与炎症因子IL-8、IL-1β及A/t存在相关性（P<0.05）；多巴胺质量浓度与肺功能的FRC、TLC、FEV₅₀/FVC，FEV₁₀₀/FVC呈负相关（P<0.05）。结论针刺能够缓解COPD大鼠炎症状态，改善肺功能，提高血浆多巴胺水平，且针刺对COPD大鼠肺功能的影响可能与降低炎症因子及提高多巴胺水平相关。

英文摘要:

ObjectiveTo explore the mechanism of acupuncture in regulating chronic inflammation through dopamine in rats with chronic obstructive pulmonary disease (COPD). Methods32 SD rats were randomly divided into control, model, sham acupuncture and acupuncture groups （n=8）. COPD condition was induced by eight-week exposure to cigarette smoking and lipopolysaccharides (LPS) of the rats, except for those in the control group. From the beginning of the 7th week, the acupuncture group received bilateral electroacupuncture on the Zusanli (ST-36), while the sham acupuncture group received bilateral electroacupuncture on the non-points, 30 min/time, 1/day, for 2 weeks prior to exposure to cigarette smoking. Post treatment changes in plasma dopamine and inflammatory factors 〔tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8)〕, lung function 〔total lung capacity (TLC), functional residual capacity (FRC), the 50 μs forced expiratory volume (FEV) vs. forced vital capacity (FVC)( FEV₅₀/FVC), the 100 μs FEV vs. FVC (FEV₁₀₀/FVC), total airway resistance (RL), lung dynamic compliance (Cdyn)〕, and the ratio of total alveolus area to tissue area (A/t) and cell counts in the alveolar lavage fluid (BALF) were measured. Pearson correlations between plasma dopamine and the above indicators were calculated. ResultsAcupuncture increased plasma dopamine and improved the inflammatory factors, lung function, A/t and BALF cell counts. Compared with the model rats, the rats that received acupuncture had higher levels of TNF-α and IL-1β, A/t and BALF cell counts, and lung function (FEV₅₀/FVC, FEV₁₀₀/FVC, RL, Cdyn) (P<0.05). The effects of acupuncture were superior on the ST-36 points compared with the non-points. Significant correlations between lung function (FRC, RL, Cdyn) and inflammatory factors (TNF-α, IL-1β, IL-6, IL-8) were found (P<0.001）. TLC was correlated with IL-8, IL-1β and A/t (P<0.05). Plasma dopamine was correlated with FRC, TLC, FEV₅₀/FVC, FEV₁₀₀/FVC (P<0.05). ConclusionAcupuncture can alleviate inflammation, improve lung function and raise plasma dopamine level in COPD rats, and the effect of acupuncture on lung function may be related to reducing inflammatory factors and increasing dopamine level.

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