胡大敏, 沈富兵, 谭雪玲, 等.α-苦瓜素对人正常肝细胞细胞因子表达的调节作用.四川大学学报(医学版),2019,50(2):193-196
α-苦瓜素对人正常肝细胞细胞因子表达的调节作用
Alpha-momordicin Regulates Hepatocyte Cytokine Expression
  
中文关键词:  α-苦瓜素肝细胞L02细胞因子调节作用
英文关键词:α-momordicinLiver cell L02CytokineRegulation
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中文摘要:
      目的探索α-苦瓜素(α-MMC)对肝细胞细胞因子合成与分泌的调节作用。方法以质量浓度为189 μg/mL的α-MMC加入体外培养的肝细胞L02中,分别于药物作用0 h、2 h、4 h和8 h后收获培养上清和细胞裂解液样品,以Bio-Plex 200悬液芯片检测系统检测各样品中辅助性T细胞17(TH17)细胞因子谱的表达量。结果与0 h相比,α-MMC作用L02肝细胞2 h、4 h和8 h后,细胞内合成的白介素(IL)-1b、IL-6、IL-17A、IL-31、IL-33、可溶性CD40配体(sCD40L)、肿瘤坏死因子-α(TNF-α)等7种细胞因子降低(P<0.05),分泌至细胞外液的IL-6、IL-4、IL-17A和sCD40L也降低(P<0.05)。结论α-MMC能抑制肝细胞IL-6、IL-17A和TNF-α等细胞因子的合成与分泌,可能在抗肿瘤的应用中产生副作用。
英文摘要:
      ObjectiveTo investigate the regulation effect of α-momordicin (α-MMC) on the synthesis and secretion of cytokines in hepatocytes cells.MethodsHepatocytes L02 were treated with 189 μg/mL α-MMC with culture supernatant and lysate samples were harvested in different timepoint. Expressions of T-helper 17 (TH17) cytokine profile in samples were detected by the Bio-Plex 200 suspension chip assay system. ResultsCompared with 0 h, after the α-MMC treatment of L02 hepatocytes for 2 h, 4 h and 8 h, the intracellular synthesis of cytokines interleukin (IL)-1b, IL-6, IL-17A, IL-31, IL-33, soluble CD40 ligand (sCD40L), tumor necrosis factor-α (TNF-α) were all significantly decreased (P<0.05), and IL-6, IL-4, IL-17A, and sCD40L secreted into the extracellular fluid also decreased significantly (P<0.05). Conclusionα-MMC can significantly inhibit the synthesis and secretion of cytokines such as IL-6, IL-17A and TNF-α in hepatocytes, which may become a side effect of its anti-tumor application.
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