TWEAK/Fn14蛋白在胰腺癌组织中的表达

目的通过检测并比较肿瘤坏死因子样凋亡弱诱导剂（TWEAK）和人成纤维细胞生长因子诱导型14（Fn14）在正常胰腺组织、慢性胰腺炎及胰腺癌组织中的表达，分析探讨TWEAK/Fn14通路在胰腺癌发展过程中的作用。方法收集在四川大学华西医院胰腺外科行手术治疗、切除的胰腺组织标本，并经病理检察证实，胰腺癌标本50例，慢性胰腺炎标本40例，正常胰腺组织标本30例。采用免疫组化检测胰腺组织中TWEAK和Fn14的表达，并分析TWEAK表达与胰腺癌临床病理特征的关系。结果 TWEAK阳性表达率在胰腺癌为36.0%（18/50），高于慢性胰腺炎17.5%（7/40）及正常胰腺组织13.3%（4/30），组间差异有统计学意义（ P<0.05），表达强度亦为胰腺癌组织＞慢性胰腺炎＞正常胰腺组织（ P<0.05）。Fn14在胰腺癌组织（4.0%）和正常胰腺组织（7.0%）阳性表达率低，胰腺炎组织中未发现阳性表达。TWEAK阳性表达率和高表达率在Ⅱ期患者中随着肿瘤疾病分级增加而增加（ P<0.05）。而各病理分级中，TWEAK阳性表达率、高表达率和EI评分的差异均无统计学意义。结论 TWEAK/Fn14参与了胰腺癌的发生发展过程。TWEAK在胰腺癌中高表达，Fn14呈低表达。

英文摘要:

Objective To investigate the effect of tumor necrosis factor -related weak inducer of apoptosis (TWEAK)/fibroblast growth factor-inducible 14 (Fn14) on the proliferation and growth of pancreatic cancer. Methods Human pancreatic cancer (50 cases), chronic pancreatitis (40 cases) and normal pancreatic tissues (30 cases) were collected in West China Hospital from January 2012 to December 2013. TWEAK and Fn14 expressions in these tissues were checked with hematoxylin-eosin (HE) staining and immunohistochemistry method. Relationship between TWEAK expression and clinicopathological features of pancreatic cancer was analyzed. Results TWEAK expression rate was 36% (18/50) in pancreatic cancer, higher than that in chronic pancreatitis (17.5%, 7/40) and normal pancreatic tissues (13.3%, 4/30) ( P<0.05) .Expression intensity of TWEAK in three groups was also obviously ( P<0.05). The expression rate of Fn14 was 4.0% in pancreatic cancer ,7.0% in normal pancreatic tissues ,and 0% in chronic pancreatitis. TWEAK positive expression rate and high expression rate in pancreatic cancer were higher in IIB group ( P<0.05). Pathological grade was not related to TWEAK expression. Conclusion TWEAK/Fn14 involved in the progression of pancreatic cancer. In the tissue of pancreatic cancer, TWEAK was highly expressed, and Fn14 was lowly expressed.

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