哮喘伴抑郁患者CD4<sup>+</sup> T淋巴细胞五羟色胺受体及转运体基因的表达

目的探索哮喘和抑郁二者共病的可能机制。方法使用自制量表、哮喘控制测试表（ACT）、哮喘生存质量问卷（AQLQ）、汉密尔顿抑郁量表（HRSD）对受试者（分为哮喘组、哮喘伴抑郁组、正常对照组）的一般情况及哮喘严重程度、哮喘控制情况、哮喘生活质量、心理卫生情况进行评估。对哮喘及哮喘伴抑郁组受试者测定肺功能及血中嗜酸性粒细胞及IgE水平。所有受试者的血样提取分选CD4⁺ T细胞，行实时荧光定量聚合酶链式反应（real time-PCR）检测SLC6A4、HTR7、HTR1A、HTR2A基因的表达水平。结果哮喘组及哮喘伴抑郁组SLC6A4基因的相对表达量低于正常对照组( P=0.000)；哮喘组HTR2A基因的表达量低于正常对照组( P=0.021)，哮喘组HTR2A基因表达有低于哮喘伴抑郁组的趋势( P=0.077)；哮喘伴抑郁组较哮喘组有更低的哮喘生活质量评分( P=0.004)。结论支气管哮喘及抑郁可能存在基因水平的联系，SLC6A4基因的表达水平下降可能是二者共病的机制之一。

英文摘要:

Objective To explore the comorbidity mechanism of asthma and depression. Methods A self-developed questionnaire, which also contained Asthma Control Test (ACT), Asthma Quality of Life Questionnaire (AQLQ) and Hamilton Rating Scale for depression (HRSD), was administered in 41 participants with asthma (AS), asthma and depression (AD), or none any of these conditions (health control, HC). Lung function and blood levels of eosnophils and IgE were also detected in those in the AS and AD groups. Blood CD4⁺ T cells were isolated in all of the participants to extract RNA for reverse transcription to cDNA. Real-time polymerase chain reaction (RT-PCR) was performed using cDNA as a template to detected the expression levels of HTR1A, HTR2A, HTR7, SLC6A4, and B₂M genes. Results Participants with AS and AD had lower expression level ofSLC6A4 than the healthy controls ( P=0.000). The expression level of HTR2Ain participants with AS was lower than that in the healthy controls ( P=0.021) and marginally lower than that in participants with AD ( P=0.077). Participants with AD had lower AQLQ scores than participants with AS ( P=0.004). Conclusion Asthma and depression is correlated at gene level. Decreased expression ofSLC6A4gene may be one of the possible comorbidity mechanisms of asthma and depression.

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