目的 探讨氢气对大鼠肾脏缺血再灌注损伤的保护作用及其机制。方法 将18只成年雄性SD大鼠随机分成假手术(Sham)组、缺血再灌注(I/R)组、氢气治疗（H2)组3组，每组6只。I/R组大鼠右肾切除术后，制备左肾缺血再灌注损伤模型；Sham组大鼠右肾切除后，仅分离而不夹闭左肾肾蒂；H2组从再灌注前10 min开始吸入浓度为2.5%的氢气130 min，其余操作与I/R组相同。比较再灌注24 h 3组之间血清肌酐(Cr)、尿素氮(BUN)，肾组织丙二醛(MDA)水平以及肾小管损伤的组织病理学半定量评分。结果 I/R可导致大鼠肾脏严重损伤。吸入浓度为2.5%的氢气可以降低大鼠BUN、Cr和MDA水平（P<0.05）；同时，H2组肾组织病理学评分低于I/R组（P<0.05）。结论 吸入低浓度的氢气对肾脏缺血再灌注损伤有保护作用，其机制与减少肾组织中MDA的生成有关。
英文摘要:
Objective?To investigate the protective effects of hydrogen on renal ischemic/reperfusion injury and its mechanism in rats. Methods?Eighteen adult male Sprague-Dawley rats were used and allocated into sham-operated group (Sham group), ischemic reperfusion group (I/R group) and hydrogen treated group (H2 group) randomly. Ischemic/reperfusion of the left renal pedicles following a right nephrectomy was investigated in I/R group. In the Sham group, the rats were subjected to identical surgical procedure without occlusion of left renal pedicles. In the H2 group, inhalation of the 2.5% hydrogen was initiated 10 min before reperfusion and last for 130 min till the end of the 120-min reperfusion. Plasma level of usea nitrogen (BUN), creatinine (Cr), maiondiaidehyde (MDA), the score of histopathological examination of left kidney were evaluated 24 h after operation in all subjects. Results?Ischemia-reperfusion injury rat model was established successfully. Inhalation of hydrogen (2.5%) markedly reduced the level of serum BUN, CREA and MDA (P<0.05). The histopathological outcome in the H2 group was significantly improved, compared to the I/R group (P<0.05). Conclusion?Inhalation of hydrogen could attenuate renal ischemic/reperfusion injury in rats by decreasing the content of MDA.
