Bcl-2、PD-L1在大肠癌组织中的表达及意义

【摘要】目的探讨结肠癌组织中凋亡抑制基因Bcl-2蛋白、免疫共刺激分子PD-L1蛋白的表达及其与肿瘤转移的关系。方法应用免疫组化技术检测57例新鲜大肠癌组织及其癌旁正常组织中Bcl-2、PD-L1的表达，分析Bcl-2、PD-L1表达与大肠癌患者临床病理特征的关系。结果大肠癌组织中Bcl-2蛋白阳性表达率为75.43%，而癌旁正常组织中阳性表达率为52.63%，两者差异具有统计学意义（P<0.05）；大肠癌组织中有PD-L1蛋白的原位表达，阳性表达率为45.61%，而癌旁正常组织PD-L1蛋白阳性表达率为15.79%，两者差异具有统计学意义（P<0.01）；Bcl-2的表达率和表达强度与病理学分化程度有关，高-中分化大肠癌患者癌组织中Bcl-2的表达率和表达强度高于低分化者，差异有统计学意义（P<0.05）； PD-L1蛋白的阳性表达率与大肠癌的淋巴结转移有关(P<0.05)，而与浸润深度无关。在高-中分化组、Dukes分期A+B组、无淋巴结转移组中Bcl-2的阳性表达率高于PD-L1（P<0.05），而在低分化组、Dukes分期C+D组、有淋巴结转移组中二者表达率无明显差异（P>0.05）。结论 Bcl-2 可能与结肠癌的病理学分化程度有关，PD-L1可能与结肠癌的转移发展有关；共检测Bcl-2和PD-L1可能预测大肠癌的病理分化程度、Dukes分期及有无淋巴结转移。

英文摘要:

【Abstract】 Objective To study the expression of Bcl-2 and PD-L1 in colon cancer and its relationship with metastasis. Methods Tumor samples were collected from 57 cases of colon cancer, and tumor adjacent normal mucous tissue were obtained as normal control. The expressions of Bcl-2 and PD-L1 were assayed by immunohistochemical staining, and its correlations with patients’ clinical pathological indexes were analyzed. Results The positive rate of Bcl-2 protein was 75.43% and 52.63% in colon cancer tissues and tumor adjacent colon mucous tissue respectively, with statistically significant difference (P<0.05). While the positive rate of PD-L1 was 45.61% and 15.79% in colon cancer tissues and tumor adjacent colon mucous tissue respectively, also with signficant difference (P<0.01). The positive rate of Bcl-2 was correlated with cellular differentiation, and the positive rate of PD-L1 protein was correlated with lymph node metastasis (P<0.05). Conclusion The expression of Bcl-2 may be associated with colon cancer pathologic differentiation, and the expression of PD-L1 may be associated with colon cancer metastasis.

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